Human CYP27B1 ELISA Kit

Alternative name
Human 25-OHD-1 alpha-hydroxylase ELISA Kit;Human 25-hydroxyvitamin D(3) 1-alpha-hydroxylase ELISA Kit;Human VD3 1A hydroxylase ELISA Kit;Human calcidiol 1-monooxygenase ELISA Kit;Human cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit;Human cytochrome P450C1 alpha ELISA Kit;Human cytochrome P450VD1-alpha ELISA Kit;Human cytochrome p450 27B1 ELISA Kit;Human CYP1alpha ELISA Kit;Human CYP27B ELISA Kit;Human CP2B ELISA Kit;Human CYP1 ELISA Kit;Human P450c1 ELISA Kit;Human PDDR ELISA Kit;Human VDD1 ELISA Kit;Human VDDR ELISA Kit;Human VDDRI ELISA Kit;Human VDR ELISA Kit;Human cytochrome P450 family 27 subfamily B member 1 ELISA Kit;Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial ELISA Kit;Human 1alpha(OH)ase ELISA Kit;Human 25 hydroxyvitamin D3-1-alpha hydroxylase ELISA Kit;Human cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit;
Catalog
E011120
species
Human
GeneCYP27B1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CYP27B1. No significant cross-reactivity or interference between human CYP27B1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity7.81 pg/ml.
Intended UseHuman CYP27B1 ELISA Kit allows for the in vitro quantitative determination of CYP27B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP27B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP27B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP27B1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP27B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial Protein information
Uniprot ID CP27B_HUMAN
Uniprot AC O15528; B2RC61; Q548T3;
UniGene Hs.524528;
GeneID 1594
KEGG hsa:1594;
Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial Protein SEQUENCE
SEQUENCE 508 AA; 56504 MW; 7F0611EFAD1B5C1C CRC64;
MTQTLKYASR VFHRVRWAPE LGASLGYREY HSARRSLADI PGPSTPSFLA
ELFCKGGLSR LHELQVQGAA HFGPVWLASF GTVRTVYVAA PALVEELLRQ
EGPRPERCSF SPWTEHRRCR QRACGLLTAE GEEWQRLRSL LAPLLLRPQA
AARYAGTLNN VVCDLVRRLR RQRGRGTGPP ALVRDVAGEF YKFGLEGIAA
VLLGSRLGCL EAQVPPDTET FIRAVGSVFV STLLTMAMPH WLRHLVPGPW
GRLCRDWDQM FAFAQRHVER REAEAAMRNG GQPEKDLESG AHLTHFLFRE
ELPAQSILGN VTELLLAGVD TVSNTLSWAL YELSRHPEVQ TALHSEITAA
LSPGSSAYPS ATVLSQLPLL KAVVKEVLRL YPVVPGNSRV PDKDIHVGDY
IIPKNTLVTL CHYATSRDPA QFPEPNSFRP ARWLGEGPTP HPFASLPFGF
GKRSCMGRRL AELELQMALA QILTHFEVQP EPGAAPVRPK TRTVLVPERS
INLQFLDR
UCSC uc001spz.2; human.;

Category

Human CYP27B1 ELISA Kit