Human CYP3A4 ELISA Kit

Alternative name
Human 1,8-cineole 2-exo-monooxygenase ELISA Kit;Human albendazole monooxygenase ELISA Kit;Human albendazole sulfoxidase ELISA Kit;Human CYPIIIA3 ELISA Kit;Human CYPIIIA4 ELISA Kit;Human cholesterol 25-hydroxylase ELISA Kit;Human cytochrome P450 3A3 ELISA Kit;Human cytochrome P450 HLp ELISA Kit;Human cytochrome P450 NF-25 ELISA Kit;Human cytochrome P450-PCN1 ELISA Kit;Human nifedipine oxidase ELISA Kit;Human quinine 3-monooxygenase ELISA Kit;Human taurochenodeoxycholate 6-alpha-hydroxylase ELISA Kit;Human CYP3A3 ELISA Kit;Human CP33 ELISA Kit;Human CP34 ELISA Kit;Human CYP3A ELISA Kit;Human HLP ELISA Kit;Human NF-25 ELISA Kit;Human P450C3 ELISA Kit;Human P450PCN1 ELISA Kit;Human cytochrome P450 family 3 subfamily A member 4 ELISA Kit;Human cytochrome P450 3A4 ELISA Kit;Human P450-III, steroid inducible ELISA Kit;Human cytochrome P450, family 3, subfamily A, polypeptide 4 ELISA Kit;Human cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3 ELISA Kit;Human cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 ELISA Kit;Human glucocorticoid-inducible P450 ELISA Kit;
Catalog
E011077
species
Human
GeneCYP3A4
Standard Curve
Other Species Mouse CYP3A4 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 3A4 (CYP3A4). No significant cross-reactivity or interference between Cytochrome P450 3A4 (CYP3A4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.118ng/mL.
Intended UseHuman CYP3A4 ELISA Kit allows for the in vitro quantitative determination of CYP3A4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A4 (CYP3A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 3A4 (CYP3A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A4 (CYP3A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 3A4 (CYP3A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome P450 3A4 Protein information
Uniprot ID CP3A4_HUMAN
Uniprot AC P08684; P05184; Q16757; Q9UK50;
UniGene Hs.728751;
GeneID 1576
KEGG hsa:1576;
Human Cytochrome P450 3A4 Protein SEQUENCE
SEQUENCE 503 AA; 57343 MW; 38ED122BF89F74F7 CRC64;
MALIPDLAME TWLLLAVSLV LLYLYGTHSH GLFKKLGIPG PTPLPFLGNI
LSYHKGFCMF DMECHKKYGK VWGFYDGQQP VLAITDPDMI KTVLVKECYS
VFTNRRPFGP VGFMKSAISI AEDEEWKRLR SLLSPTFTSG KLKEMVPIIA
QYGDVLVRNL RREAETGKPV TLKDVFGAYS MDVITSTSFG VNIDSLNNPQ
DPFVENTKKL LRFDFLDPFF LSITVFPFLI PILEVLNICV FPREVTNFLR
KSVKRMKESR LEDTQKHRVD FLQLMIDSQN SKETESHKAL SDLELVAQSI
IFIFAGYETT SSVLSFIMYE LATHPDVQQK LQEEIDAVLP NKAPPTYDTV
LQMEYLDMVV NETLRLFPIA MRLERVCKKD VEINGMFIPK GVVVMIPSYA
LHRDPKYWTE PEKFLPERFS KKNKDNIDPY IYTPFGSGPR NCIGMRFALM
NMKLALIRVL QNFSFKPCKE TQIPLKLSLG GLLQPEKPVV LKVESRDGTV
SGA

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Human CYP3A4 ELISA Kit