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Human CYP2E1 ELISA Kit

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  • Alternative name

    Human 4-nitrophenol 2-hydroxylase ELISA Kit;Human CYPIIE1 ELISA Kit;Human cytochrome P450-J ELISA Kit;Human CYP2E ELISA Kit;Human CPE1 ELISA Kit;Human P450-J ELISA Kit;Human P450C2E ELISA Kit;Human cytochrome P450 family 2 subfamily E member 1 ELISA Kit;Human cytochrome P450 2E1 ELISA Kit;Human cytochrome P450, family 2, subfamily E, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
  • Catalog
    E011064
  • species
    Human
  • GeneCYP2E1
  • Standard CurveHuman CYP2E1 ELISA Kit
  • Other Species Mouse Cyp2e1 ELISA KitMonkey CYP2E1 ELISA KitRat Cyp2e1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2E1 (CYP2E1). No significant cross-reactivity or interference between Cytochrome P450 2E1 (CYP2E1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.31ng/mL.
  • Intended UseHuman CYP2E1 ELISA Kit allows for the in vitro quantitative determination of CYP2E1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1 (CYP2E1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2E1 (CYP2E1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2E1 (CYP2E1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2E1 (CYP2E1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytochrome P450 2E1, N-terminally processed Protein information
  • Uniprot ID CP2E1_HUMAN
  • Uniprot AC P05181; Q5VZD5; Q6NWT9; Q9UK47;
  • UniGene Hs.12907;
  • GeneID 1571
  • KEGG hsa:1571;
  • Human Cytochrome P450 2E1, N-terminally processed Protein SEQUENCE

  • SEQUENCE 493 AA; 56849 MW; ED0399E32A005644 CRC64;

    MSALGVTVAL LVWAAFLLLV SMWRQVHSSW NLPPGPFPLP IIGNLFQLEL

    KNIPKSFTRL AQRFGPVFTL YVGSQRMVVM HGYKAVKEAL LDYKDEFSGR

    GDLPAFHAHR DRGIIFNNGP TWKDIRRFSL TTLRNYGMGK QGNESRIQRE

    AHFLLEALRK TQGQPFDPTF LIGCAPCNVI ADILFRKHFD YNDEKFLRLM

    YLFNENFHLL STPWLQLYNN FPSFLHYLPG SHRKVIKNVA EVKEYVSERV

    KEHHQSLDPN CPRDLTDCLL VEMEKEKHSA ERLYTMDGIT VTVADLFFAG

    TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRIPA IKDRQEMPYM

    DAVVHEIQRF ITLVPSNLPH EATRDTIFRG YLIPKGTVVV PTLDSVLYDN

    QEFPDPEKFK PEHFLNENGK FKYSDYFKPF STGKRVCAGE GLARMELFLL

    LCAILQHFNL KPLVDPKDID LSPIHIGFGC IPPRYKLCVI PRS

  • UCSC uc001lnj.1; human.;


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