Human CYP2C9 ELISA Kit

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  • Alternative name

    Human (R)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 7-monooxygenase ELISA Kit;Human CYPIIC9 ELISA Kit;Human Cholesterol 25-hydroxylase ELISA Kit;Human cytochrome P-450MP ELISA Kit;Human Cytochrome P450 MP-4 ELISA Kit;Human Cytochrome P450 MP-8 ELISA Kit;Human cytochrome P450 PB-1 ELISA Kit;Human S-mephenytoin 4-hydroxylase ELISA Kit;Human CYP2C10 ELISA Kit;Human CPC9 ELISA Kit;Human CYP2C ELISA Kit;Human P450IIC9 ELISA Kit;Human cytochrome P450 family 2 subfamily C member 9 ELISA Kit;Human cytochrome P450 2C9 ELISA Kit;Human cytochrome P-450 S-mephenytoin 4-hydroxylase ELISA Kit;Human cytochrome P450, family 2, subfamily C, polypeptide 9 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;

  • Catalog
    E011057
  • species
    Human
  • GeneCYP2C9
  • Standard CurveHuman CYP2C9 ELISA Kit
  • Other Species Mouse CYP2C9 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2C9 (CYP2C9). No significant cross-reactivity or interference between Cytochrome P450 2C9 (CYP2C9) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.253ng/mL.
  • Intended UseHuman CYP2C9 ELISA Kit allows for the in vitro quantitative determination of CYP2C9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C9 (CYP2C9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2C9 (CYP2C9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C9 (CYP2C9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2C9 (CYP2C9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cytochrome P450 2C9 Protein information
  • Uniprot ID CP2C9_HUMAN
  • Uniprot AC P11712; P11713; Q16756; Q16872; Q5VX92; Q6IRV8; Q8WW80;
  • UniGene Hs.282624;
  • GeneID 1559
  • KEGG hsa:1559;
  • Human Cytochrome P450 2C9 Protein SEQUENCE
  • SEQUENCE 490 AA; 55628 MW; 4FDFC395303A4E3E CRC64;

    MDSLVVLVLC LSCLLLLSLW RQSSGRGKLP PGPTPLPVIG NILQIGIKDI

    SKSLTNLSKV YGPVFTLYFG LKPIVVLHGY EAVKEALIDL GEEFSGRGIF

    PLAERANRGF GIVFSNGKKW KEIRRFSLMT LRNFGMGKRS IEDRVQEEAR

    CLVEELRKTK ASPCDPTFIL GCAPCNVICS IIFHKRFDYK DQQFLNLMEK

    LNENIKILSS PWIQICNNFS PIIDYFPGTH NKLLKNVAFM KSYILEKVKE

    HQESMDMNNP QDFIDCFLMK MEKEKHNQPS EFTIESLENT AVDLFGAGTE

    TTSTTLRYAL LLLLKHPEVT AKVQEEIERV IGRNRSPCMQ DRSHMPYTDA

    VVHEVQRYID LLPTSLPHAV TCDIKFRNYL IPKGTTILIS LTSVLHDNKE

    FPNPEMFDPH HFLDEGGNFK KSKYFMPFSA GKRICVGEAL AGMELFLFLT

    SILQNFNLKS LVDPKNLDTT PVVNGFASVP PFYQLCFIPV




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