Human CYP2C19 ELISA Kit

Alternative name
Human (R)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 6-monooxygenase ELISA Kit;Human (S)-limonene 7-monooxygenase ELISA Kit;Human CYPIIC17 ELISA Kit;Human CYPIIC19 ELISA Kit;Human cytochrome P450-11A ELISA Kit;Human cytochrome P450-254C ELISA Kit;Human mephenytoin 4-hydroxylase ELISA Kit;Human CPCJ ELISA Kit;Human CYP2C ELISA Kit;Human P450C2C ELISA Kit;Human P450IIC19 ELISA Kit;Human cytochrome P450 family 2 subfamily C member 19 ELISA Kit;Human cytochrome P450 2C19 ELISA Kit;Human S-mephenytoin 4-hydroxylase ELISA Kit;Human cytochrome P-450 II C ELISA Kit;Human cytochrome P450, family 2, subfamily C, polypeptide 19 ELISA Kit;Human cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 19 ELISA Kit;Human flavoprotein-linked monooxygenase ELISA Kit;Human mephenytoin 4'-hydroxylase ELISA Kit;Human microsomal monooxygenase ELISA Kit;Human xenobiotic monooxygenase ELISA Kit;
Catalog
E011050
species
Human
GeneCYP2C19
Other Species Mouse CYP2C19 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman CYP2C19 ELISA Kit allows for the in vitro quantitative determination of CYP2C19 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CYP2C19 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CYP2C19 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CYP2C19 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CYP2C19 can be calculated.
Human Cytochrome P450 2C19 Protein information
Uniprot ID CP2CJ_HUMAN
Uniprot AC P33261; P33259; Q8WZB1; Q8WZB2; Q9UCD4;
UniGene Hs.282409;
GeneID 1557
KEGG hsa:1557;
Human Cytochrome P450 2C19 Protein SEQUENCE
SEQUENCE 490 AA; 55931 MW; 17DB04C50A132C53 CRC64;
MDPFVVLVLC LSCLLLLSIW RQSSGRGKLP PGPTPLPVIG NILQIDIKDV
SKSLTNLSKI YGPVFTLYFG LERMVVLHGY EVVKEALIDL GEEFSGRGHF
PLAERANRGF GIVFSNGKRW KEIRRFSLMT LRNFGMGKRS IEDRVQEEAR
CLVEELRKTK ASPCDPTFIL GCAPCNVICS IIFQKRFDYK DQQFLNLMEK
LNENIRIVST PWIQICNNFP TIIDYFPGTH NKLLKNLAFM ESDILEKVKE
HQESMDINNP RDFIDCFLIK MEKEKQNQQS EFTIENLVIT AADLLGAGTE
TTSTTLRYAL LLLLKHPEVT AKVQEEIERV VGRNRSPCMQ DRGHMPYTDA
VVHEVQRYID LIPTSLPHAV TCDVKFRNYL IPKGTTILTS LTSVLHDNKE
FPNPEMFDPR HFLDEGGNFK KSNYFMPFSA GKRICVGEGL ARMELFLFLT
FILQNFNLKS LIDPKDLDTT PVVNGFASVP PFYQLCFIPV
UCSC uc010qnz.3; human.;

Category

Human CYP2C19 ELISA Kit