Human AGE ELISA Kit

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  • Catalog
    E001104
  • species
    Human
  • GeneAGE
  • Other Species Human CML-AGE ELISA KitHuman GTE-AGE ELISA KitHuman Hb AGE ELISA KitHuman HB-AGE ELISA KitMouse AGE ELISA KitMouse CML-AGE ELISA KitMouse Hb-AGE ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 2.813ng/ml
  • Intended UseHuman AGE ELISA Kit allows for the in vitro quantitative determination of AGE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGE. During the reaction,Human AGE in the sample or standard competes with a fixed amount of biotin-labeled Human AGE for sites on a precoated Monoclonal antibody specific to Human AGE. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human AGE in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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