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Human CYP27A1 ELISA Kit

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  • Alternative name

    Human 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase ELISA Kit;Human cytochrome P-450C27/25 ELISA Kit;Human cytochrome P450 27 ELISA Kit;Human sterol 27-hydroxylase ELISA Kit;Human vitamin D(3) 25-hydroxylase ELISA Kit;Human CYP27 ELISA Kit;Human CP27 ELISA Kit;Human CTX ELISA Kit;Human cytochrome P450 family 27 subfamily A member 1 ELISA Kit;Human sterol 26-hydroxylase, mitochondrial ELISA Kit;Human 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase ELISA Kit;Human 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase ELISA Kit;Human cholestanetriol 26-monooxygenase ELISA Kit;Human cytochrome P450, family 27, subfamily A, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1 ELISA Kit;
  • Catalog
    E011034
  • species
    Human
  • GeneCYP27A1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27A1 (CYP27A1). No significant cross-reactivity or interference between Cytochrome P450 27A1 (CYP27A1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman CYP27A1 ELISA Kit allows for the in vitro quantitative determination of CYP27A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sterol 26-hydroxylase, mitochondrial Protein information
  • Uniprot ID CP27A_HUMAN
  • Uniprot AC Q02318; A8K303; Q6LDB4; Q86YQ6;
  • UniGene Hs.516700;
  • GeneID 1593
  • KEGG hsa:1593;
  • Human Sterol 26-hydroxylase, mitochondrial Protein SEQUENCE

  • SEQUENCE 531 AA; 60235 MW; 62025EB670DBD8E9 CRC64;

    MAALGCARLR WALRGAGRGL CPHGARAKAA IPAALPSDKA TGAPGAGPGV

    RRRQRSLEEI PRLGQLRFFF QLFVQGYALQ LHQLQVLYKA KYGPMWMSYL

    GPQMHVNLAS APLLEQVMRQ EGKYPVRNDM ELWKEHRDQH DLTYGPFTTE

    GHHWYQLRQA LNQRLLKPAE AALYTDAFNE VIDDFMTRLD QLRAESASGN

    QVSDMAQLFY YFALEAICYI LFEKRIGCLQ RSIPEDTVTF VRSIGLMFQN

    SLYATFLPKW TRPVLPFWKR YLDGWNAIFS FGKKLIDEKL EDMEAQLQAA

    GPDGIQVSGY LHFLLASGQL SPREAMGSLP ELLMAGVDTT SNTLTWALYH

    LSKDPEIQEA LHEEVVGVVP AGQVPQHKDF AHMPLLKAVL KETLRLYPVV

    PTNSRIIEKE IEVDGFLFPK NTQFVFCHYV VSRDPTAFSE PESFQPHRWL

    RNSQPATPRI QHPFGSVPFG YGVRACLGRR IAELEMQLLL ARLIQKYKVV

    LAPETGELKS VARIVLVPNK KVGLQFLQRQ C

  • UCSC uc002viz.5; human.;



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