Human CYP24A1 ELISA Kit

Alternative name
Human 24-OHase ELISA Kit;Human vitamin D(3) 24-hydroxylase ELISA Kit;Human cytochrome P450 24A1 ELISA Kit;Human cytochrome P450-CC24 ELISA Kit;Human CYP24 ELISA Kit;Human CP24 ELISA Kit;Human HCAI ELISA Kit;Human HCINF1 ELISA Kit;Human P450-CC24 ELISA Kit;Human cytochrome P450 family 24 subfamily A member 1 ELISA Kit;Human 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial ELISA Kit;Human 1,25-@dihydroxyvitamin D3 24-hydroxylase ELISA Kit;Human cytochrome P450, family 24, subfamily A, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase) ELISA Kit;Human exo-mitochondrial protein ELISA Kit;Human vitamin D 24-hydroxylase ELISA Kit;
Catalog
E011027
species
Human
GeneCYP24A1
Standard Curve
Other Species Mouse Cyp24a1 ELISA Kit Rat Cyp24a1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome P450 24A1 (CYP24A1). No significant cross-reactivity or interference between Cytochrome P450 24A1 (CYP24A1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman CYP24A1 ELISA Kit allows for the in vitro quantitative determination of CYP24A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 24A1 (CYP24A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 24A1 (CYP24A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 24A1 (CYP24A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 24A1 (CYP24A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial Protein information
Uniprot ID CP24A_HUMAN
Uniprot AC Q07973; Q15807; Q32ML3; Q5I2W7;
UniGene Hs.89663;
GeneID 1591
KEGG hsa:1591;
Human 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial Protein SEQUENCE
SEQUENCE 514 AA; 58875 MW; 8862F63771981195 CRC64;
MSSPISKSRS LAAFLQQLRS PRQPPRLVTS TAYTSPQPRE VPVCPLTAGG
ETQNAAALPG PTSWPLLGSL LQILWKGGLK KQHDTLVEYH KKYGKIFRMK
LGSFESVHLG SPCLLEALYR TESAYPQRLE IKPWKAYRDY RKEGYGLLIL
EGEDWQRVRS AFQKKLMKPG EVMKLDNKIN EVLADFMGRI DELCDERGHV
EDLYSELNKW SFESICLVLY EKRFGLLQKN AGDEAVNFIM AIKTMMSTFG
RMMVTPVELH KSLNTKVWQD HTLAWDTIFK SVKACIDNRL EKYSQQPSAD
FLCDIYHQNR LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQVQQ
KLLKEIQSVL PENQVPRAED LRNMPYLKAC LKESMRLTPS VPFTTRTLDK
ATVLGEYALP KGTVLMLNTQ VLGSSEDNFE DSSQFRPERW LQEKEKINPF
AHLPFGVGKR MCIGRRLAEL QLHLALCWIV RKYDIQATDN EPVEMLHSGT
LVPSRELPIA FCQR
UCSC uc002xwu.2; human. [Q07973-1];

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Human CYP24A1 ELISA Kit