Human SCIN ELISA Kit

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  • Alternative name

    Human scinderin ELISA Kit;Human KIAA1905 ELISA Kit;Human adseverin ELISA Kit;

  • Catalog
    E001100
  • species
    Human
  • GeneSCIN
  • Standard CurveHuman SCIN ELISA Kit
  • Other Species Mouse Scin ELISA KitBovine SCIN ELISA KitChicken SCIN ELISA KitPorcine SCIN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SCIN. No significant cross-reactivity or interference between SCIN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCIN and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 mug/mL.
  • Intended UseHuman SCIN ELISA Kit allows for the in vitro quantitative determination of SCIN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: SCIN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SCIN antibody and an SCIN-HRP conjugate. The assay sample and buffer are incubated together with SCIN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCIN concentration since SCIN from samples and SCIN-HRP conjugate compete for the anti-SCIN antibody binding site. Since the number of sites is limited, as more sites are occupied by SCIN from the sample, fewer sites are left to bind SCIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCIN concentration in each sample is interpolated from this standard curve.
  • Human Adseverin Protein information
  • Uniprot ID ADSV_HUMAN
  • Uniprot AC Q9Y6U3; A8K2U8; Q8NBZ6; Q8WU97; Q96JC7; Q96PY2;
  • UniGene Hs.633359; Hs.655515;
  • GeneID 85477
  • KEGG hsa:85477;
  • Human Adseverin Protein SEQUENCE
  • SEQUENCE 715 AA; 80489 MW; 20420C82DB2E2D24 CRC64;

    MARELYHEEF ARAGKQAGLQ VWRIEKLELV PVPQSAHGDF YVGDAYLVLH

    TAKTSRGFTY HLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQNRELQ

    GYESNDFVSY FKGGLKYKAG GVASGLNHVL TNDLTAKRLL HVKGRRVVRA

    TEVPLSWDSF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA NQVATGIRYN

    ERKGRSELIV VEEGSEPSEL IKVLGEKPEL PDGGDDDDII ADISNRKMAK

    LYMVSDASGS MRVTVVAEEN PFSMAMLLSE ECFILDHGAA KQIFVWKGKD

    ANPQERKAAM KTAEEFLQQM NYSKNTQIQV LPEGGETPIF KQFFKDWRDK

    DQSDGFGKVY VTEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGKVEI

    WRVENNGRIQ VDQNSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL

    TTSAFLTVQL DRSLGGQAVQ IRVSQGKEPV HLLSLFKDKP LIIYKNGTSK

    KGGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDV FVLKLPQNSG

    YIWVGKGASQ EEEKGAEYVA SVLKCKTLRI QEGEEPEEFW NSLGGKKDYQ

    TSPLLETQAE DHPPRLYGCS NKTGRFVIEE IPGEFTQDDL AEDDVMLLDA

    WEQIFIWIGK DANEVEKKES LKSAKMYLET DPSGRDKRTP IVIIKQGHEP

    PTFTGWFLGW DSSKW

  • UCSC uc003ssn.5; human. [Q9Y6U3-1];


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