Human COX4I1 ELISA Kit

Alternative name
Human cytochrome c oxidase polypeptide IV ELISA Kit;Human Cytochrome c oxidase subunit IV isoform 1 ELISA Kit;Human COX IV-1 ELISA Kit;Human COX4 ELISA Kit;Human COX4-1 ELISA Kit;Human COXIV ELISA Kit;Human COXIV-1 ELISA Kit;Human cytochrome c oxidase subunit 4I1 ELISA Kit;Human cytochrome c oxidase subunit 4 isoform 1, mitochondrial ELISA Kit;Human cytochrome c oxidase subunit IV ELISA Kit;
Catalog
E010993
species
Human
GeneCOX4I1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman COX4I1 ELISA Kit allows for the in vitro quantitative determination of COX4I1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Protein information
Uniprot ID COX41_HUMAN
Uniprot AC P13073; B2R4J2; D3DUM7; Q6P666;
UniGene Hs.433419;
GeneID 1327
KEGG hsa:1327;
Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Protein SEQUENCE
SEQUENCE 169 AA; 19577 MW; F6382FFDCF3BE4ED CRC64;
MLATRVFSLV GKRAISTSVC VRAHESVVKS EDFSLPAYMD RRDHPLPEVA
HVKHLSASQK ALKEKEKASW SSLSMDEKVE LYRIKFKESF AEMNRGSNEW
KTVVGGAMFF IGFTALVIMW QKHYVYGPLP QSFDKEWVAK QTKRMLDMKV
NPIQGLASKW DYEKNEWKK
UCSC uc002fje.4; human.;

Human COX4I1 ELISA Kit

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