Human COX2 ELISA Kit

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  • Alternative name

    Cytochrome c oxidase subunit 2 ELISA KIT; Shal_0258 ELISA KIT;

  • Catalog
    E010990
  • species
    Human
  • GeneCOX2
  • Standard CurveHuman COX2 ELISA Kit
  • Other Species Mouse COX2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of COX2. No significant cross-reactivity or interference between COX2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of COX2 is typically less than 0.058ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman COX2 ELISA Kit allows for the in vitro quantitative determination of COX2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to COX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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