SpecificityThis assay has high sensitivity and excellent specificity for detection of COX6C. No significant cross-reactivity or interference between COX6C and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COX6C and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman COX6C ELISA Kit allows for the in vitro quantitative determination of COX6C , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses: This COX6C ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human COX6C. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||COX6C ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COX6C antibody and an COX6C-HRP conjugate. The assay sample and buffer are incubated together with COX6C-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COX6C concentration since COX6C from samples and COX6C-HRP conjugate compete for the anti-COX6C antibody binding site. Since the number of sites is limited, as more sites are occupied by COX6C from the sample, fewer sites are left to bind COX6C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX6C concentration in each sample is interpolated from this standard curve.
Human Cytochrome c oxidase subunit 6C Protein information
Human Cytochrome c oxidase subunit 6C Protein SEQUENCE