Human COX5A ELISA Kit

Alternative name
Human cytochrome c oxidase polypeptide Va ELISA Kit;Human COX ELISA Kit;Human COX-VA ELISA Kit;Human VA ELISA Kit;Human cytochrome c oxidase subunit 5A ELISA Kit;Human cytochrome c oxidase subunit 5A, mitochondrial ELISA Kit;Human cytochrome c oxidase polypeptide, mitochondrial ELISA Kit;Human cytochrome c oxidase subunit Va ELISA Kit;Human mitochondrial cytochrome c oxidase subunit Va ELISA Kit;
Catalog
E010971
species
Human
GeneCOX5A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman COX5A ELISA Kit allows for the in vitro quantitative determination of COX5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COX5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COX5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COX5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COX5A can be calculated.
Human Cytochrome c oxidase subunit 5A, mitochondrial Protein information
Uniprot ID COX5A_HUMAN
Uniprot AC P20674; P30045; Q8TB65;
UniGene Hs.401903;
GeneID 9377
KEGG hsa:9377;
Human Cytochrome c oxidase subunit 5A, mitochondrial Protein SEQUENCE
SEQUENCE 150 AA; 16762 MW; 104DAF468BD302FC CRC64;
MLGAALRRCA VAATTRADPR GLLHSARTPG PAVAIQSVRC YSHGSQETDE
EFDARWVTYF NKPDIDAWEL RKGINTLVTY DMVPEPKIID AALRACRRLN
DFASTVRILE VVKDKAGPHK EIYPYVIQEL RPTLNELGIS TPEELGLDKV
UCSC uc002azi.5; human.;

Human COX5A ELISA Kit

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