Human CSRP3 ELISA Kit

Alternative name
Human Cardiac LIM protein ELISA Kit;Human Cysteine-rich protein 3 ELISA Kit;Human CRP3 ELISA Kit;Human LIM domain protein, cardiac ELISA Kit;Human Muscle LIM protein ELISA Kit;Human CLP ELISA Kit;Human MLP ELISA Kit;Human CMD1M ELISA Kit;Human CMH12 ELISA Kit;Human LMO4 ELISA Kit;Human cysteine and glycine rich protein 3 ELISA Kit;Human cysteine and glycine-rich protein 3 ELISA Kit;Human LIM domain only 4 ELISA Kit;Human cardiac LIM domain protein ELISA Kit;Human cysteine and glycine-rich protein 3 (cardiac LIM protein) ELISA Kit;Human muscle lim protein isoform ELISA Kit;
Catalog
E010792
species
Human
GeneCSRP3
SpecificityThis assay has high sensitivity and excellent specificity for detection of CSRP3. No significant cross-reactivity or interference between CSRP3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSRP3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CSRP3 ELISA Kit allows for the in vitro quantitative determination of CSRP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: CSRP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSRP3 antibody and an CSRP3-HRP conjugate. The assay sample and buffer are incubated together with CSRP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSRP3 concentration since CSRP3 from samples and CSRP3-HRP conjugate compete for the anti-CSRP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSRP3 from the sample, fewer sites are left to bind CSRP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSRP3 concentration in each sample is interpolated from this standard curve.
Human Cysteine and glycine-rich protein 3 Protein information
Uniprot ID CSRP3_HUMAN
Uniprot AC P50461; Q9P131; S4S7M7;
UniGene Hs.83577;
GeneID 8048
KEGG hsa:8048;
Human Cysteine and glycine-rich protein 3 Protein SEQUENCE
SEQUENCE 194 AA; 20969 MW; FDB6E4F8D258C35F CRC64;
MPNWGGGAKC GACEKTVYHA EEIQCNGRSF HKTCFHCMAC RKALDSTTVA
AHESEIYCKV CYGRRYGPKG IGYGQGAGCL STDTGEHLGL QFQQSPKPAR
SVTTSNPSKF TAKFGESEKC PRCGKSVYAA EKVMGGGKPW HKTCFRCAIC
GKSLESTNVT DKDGELYCKV CYAKNFGPTG IGFGGLTQQV EKKE
UCSC uc001mpk.4; human. [P50461-1];

Human CSRP3 ELISA Kit

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