Human CST5 ELISA Kit

Alternative name
Human Cystatin-5 ELISA Kit;Human cystatin D ELISA Kit;Human cystatin-D ELISA Kit;Human cystatin 5 ELISA Kit;Human cysteine-proteinase inhibitor ELISA Kit;
Catalog
E010768
species
Human
GeneCST5
SpecificityThis assay has high sensitivity and excellent specificity for detection of CSTD. No significant cross-reactivity or interference between CSTD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CSTD and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CST5 ELISA Kit allows for the in vitro quantitative determination of CST5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CSTD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CSTD antibody and an CSTD-HRP conjugate. The assay sample and buffer are incubated together with CSTD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSTD concentration since CSTD from samples and CSTD-HRP conjugate compete for the anti-CSTD antibody binding site. Since the number of sites is limited, as more sites are occupied by CSTD from the sample, fewer sites are left to bind CSTD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSTD concentration in each sample is interpolated from this standard curve.
Human Cystatin-D Protein information
Uniprot ID CYTD_HUMAN
Uniprot AC P28325; Q5JRF5; Q9UCA0;
UniGene Hs.121489;
GeneID 1473
KEGG hsa:1473;
Human Cystatin-D Protein SEQUENCE
SEQUENCE 142 AA; 16080 MW; CEFA89BA87A0DA68 CRC64;
MMWPMHTPLL LLTALMVAVA GSASAQSRTL AGGIHATDLN DKSVQCALDF
AISEYNKVIN KDEYYSRPLQ VMAAYQQIVG GVNYYFNVKF GRTTCTKSQP
NLDNCPFNDQ PKLKEEEFCS FQINEVPWED KISILNYKCR KV
UCSC uc002wtr.2; human.;

Human CST5 ELISA Kit

Human Cystatin-5 ELISA Kit;Human cystatin D ELISA Kit;Human cystatin-D ELISA Kit;Human cystatin 5 ELISA Kit;Human cysteine-proteinase inhibitor ELISA Kit;

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