Human CBS ELISA Kit

Alternative name
Human beta-thionase ELISA Kit;Human serine sulfhydrase ELISA Kit;Human HIP4 ELISA Kit;Human cystathionine-beta-synthase ELISA Kit;Human cystathionine beta-synthase ELISA Kit;Human methylcysteine synthase ELISA Kit;
Catalog
E010728
species
Human
GeneCBS
Standard Curve
Other Species Mouse Cbs ELISA Kit Mouse MSRB2/CBS-1/MSRB/CGI-131 ELISA Kit Monkey CBS ELISA Kit Rabbit CBS ELISA Kit Rat Cbs ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CBS. No significant cross-reactivity or interference between CBS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CBS and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CBS ELISA Kit allows for the in vitro quantitative determination of CBS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CBS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CBS antibody and an CBS-HRP conjugate. The assay sample and buffer are incubated together with CBS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CBS concentration since CBS from samples and CBS-HRP conjugate compete for the anti-CBS antibody binding site. Since the number of sites is limited, as more sites are occupied by CBS from the sample, fewer sites are left to bind CBS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CBS concentration in each sample is interpolated from this standard curve.
Human Cystathionine beta-synthase Protein information
Uniprot ID CBS_HUMAN
Uniprot AC P35520; B2R993; D3DSK4; Q99425; Q9BWC5;
UniGene Hs.533013;
GeneID 875
KEGG hsa:875;
Human Cystathionine beta-synthase Protein SEQUENCE
SEQUENCE 551 AA; 60587 MW; F89E69C67BDE6701 CRC64;
MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS
RCTWQLGRPA SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG
LKCELLAKCE FFNAGGSVKD RISLRMIEDA ERDGTLKPGD TIIEPTSGNT
GIGLALAAAV RGYRCIIVMP EKMSSEKVDV LRALGAEIVR TPTNARFDSP
ESHVGVAWRL KNEIPNSHIL DQYRNASNPL AHYDTTADEI LQQCDGKLDM
LVASVGTGGT ITGIARKLKE KCPGCRIIGV DPEGSILAEP EELNQTEQTT
YEVEGIGYDF IPTVLDRTVV DKWFKSNDEE AFTFARMLIA QEGLLCGGSA
GSTVAVAVKA AQELQEGQRC VVILPDSVRN YMTKFLSDRW MLQKGFLKEE
DLTEKKPWWW HLRVQELGLS APLTVLPTIT CGHTIEILRE KGFDQAPVVD
EAGVILGMVT LGNMLSSLLA GKVQPSDQVG KVIYKQFKQI RLTDTLGRLS
HILEMDHFAL VVHEQIQYHS TGKSSQRQMV FGVVTAIDLL NFVAAQERDQ
K
UCSC uc002zct.3; human. [P35520-1];

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Category

Human CBS ELISA Kit

Human beta-thionase ELISA Kit;Human serine sulfhydrase ELISA Kit;Human HIP4 ELISA Kit;Human cystathionine-beta-synthase ELISA Kit;Human cystathionine beta-synthase ELISA Kit;Human methylcysteine synthase ELISA Kit;