Intended UseHuman CDKN1A ELISA Kit allows for the in vitro quantitative determination of CDKN1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDKN1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CDKN1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDKN1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDKN1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Cyclin-dependent kinase inhibitor 1 Protein information
P38936; Q14010; Q6FI05; Q9BUT4;
Human Cyclin-dependent kinase inhibitor 1 Protein SEQUENCE