Human GAK ELISA Kit

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  • Alternative name

    Human DNAJ26 ELISA Kit;Human DNAJC26 ELISA Kit;Human cyclin G associated kinase ELISA Kit;Human cyclin-G-associated kinase ELISA Kit;Human auxilin-2 ELISA Kit;

  • Catalog
    E010617
  • species
    Human
  • GeneGAK
  • Standard CurveHuman GAK ELISA Kit
  • Other Species Mouse Gak ELISA KitRat Gak ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GAK. No significant cross-reactivity or interference between GAK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman GAK ELISA Kit allows for the in vitro quantitative determination of GAK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: GAK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAK antibody and an GAK-HRP conjugate. The assay sample and buffer are incubated together with GAK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAK concentration since GAK from samples and GAK-HRP conjugate compete for the anti-GAK antibody binding site. Since the number of sites is limited, as more sites are occupied by GAK from the sample, fewer sites are left to bind GAK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAK concentration in each sample is interpolated from this standard curve.
  • Human Cyclin-G-associated kinase Protein information
  • Uniprot ID GAK_HUMAN
  • Uniprot AC TQPRPNYA SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT
  • UniGene Hs.369607;
  • GeneID 2580
  • KEGG hsa:2580;
  • Human Cyclin-G-associated kinase Protein SEQUENCE
  • SEQUENCE 1311 AA; 143191 MW; 0ACE45DF57A5F981 CRC64;

    MSLLQSALDF LAGPGSLGGA SGRDQSDFVG QTVELGELRL RVRRVLAEGG

    FAFVYEAQDV GSGREYALKR LLSNEEEKNR AIIQEVCFMK KLSGHPNIVQ

    FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKKMESR GPLSCDTVLK

    IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH

    YPDYSWSAQR RALVEEEITR NTTPMYRTPE IIDLYSNFPI GEKQDIWALG

    CILYLLCFRQ HPFEDGAKLR IVNGKYSIPP HDTQYTVFHS LIRAMLQVNP

    EERLSIAEVV HQLQEIAAAR NVNPKSPITE LLEQNGGYGS ATLSRGPPPP

    VGPAGSGYSG GLALAEYDQP YGGFLDILRG GTERLFTNLK DTSSKVIQSV

    ANYAKGDLDI SYITSRIAVM SFPAEGVESA LKNNIEDVRL FLDSKHPGHY

    AVYNLSPRTY RPSRFHNRVS ECGWAARRAP HLHTLYNICR NMHAWLRQDH

    KNVCVVHCMD GRAASAVAVC SFLCFCRLFS TAEAAVYMFS MKRCPPGIWP

    SHKRYIEYMC DMVAEEPITP HSKPILVRAV VMTPVPLFSK QRSGCRPFCE

    VYVGDERVAS TSQEYDKMRD FKIEDGKAVI PLGVTVQGDV LIVIYHARST

    LGGRLQAKMA SMKMFQIQFH TGFVPRNATT VKFAKYDLDA CDIQEKYPDL

    FQVNLEVEVE PRDRPSREAP PWENSSMRGL NPKILFSSRE EQQDILSKFG

    KPELPRQPGS TAQYDAGAGS PEAEPTDSDS PPSSSADASR FLHTLDWQEE

    KEAETGAENA SSKESESALM EDRDESEVSD EGGSPISSEG QEPRADPEPP

    GLAAGLVQQD LVFEVETPAV LPEPVPQEDG VDLLGLHSEV GAGPAVPPQA

    CKAPSSNTDL LSCLLGPPEA ASQGPPEDLL SEDPLLLASP APPLSVQSTP

    RGGPPAAADP FGPLLPSSGN NSQPCSNPDL FGEFLNSDSV TVPPSFPSAH

    SAPPPSCSAD FLHLGDLPGE PSKMTASSSN PDLLGGWAAW TETAASAVAP

    TPATEGPLFS PGGQPAPCGS QASWTKSQNP DPFADLGDLS SGLQGSPAGF

    PPGGFIPKTA TTPKGSSSWQ TSRPPAQGAS WPPQAKPPPK ACTQPRPNYA

    SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT

    IAEMRKQDLA KDTDPLKLKL LDWIEGKERN IRALLSTLHT VLWDGESRWT

    PVGMADLVAP EQVKKHYRRA VLAVHPDKAA GQPYEQHAKM IFMELNDAWS

    EFENQGSRPL F

  • UCSC uc003gbm.6; human. [O14976-1];


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