Human GAK ELISA Kit

Alternative name
Human DNAJ26 ELISA Kit;Human DNAJC26 ELISA Kit;Human cyclin G associated kinase ELISA Kit;Human cyclin-G-associated kinase ELISA Kit;Human auxilin-2 ELISA Kit;
Catalog
E010617
species
Human
GeneGAK
SpecificityThis assay has high sensitivity and excellent specificity for detection of GAK. No significant cross-reactivity or interference between GAK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman GAK ELISA Kit allows for the in vitro quantitative determination of GAK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: GAK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAK antibody and an GAK-HRP conjugate. The assay sample and buffer are incubated together with GAK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAK concentration since GAK from samples and GAK-HRP conjugate compete for the anti-GAK antibody binding site. Since the number of sites is limited, as more sites are occupied by GAK from the sample, fewer sites are left to bind GAK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAK concentration in each sample is interpolated from this standard curve.
Human Cyclin-G-associated kinase Protein information
Uniprot ID GAK_HUMAN
Uniprot AC TQPRPNYA SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT
UniGene Hs.369607;
GeneID 2580
KEGG hsa:2580;
Human Cyclin-G-associated kinase Protein SEQUENCE
SEQUENCE 1311 AA; 143191 MW; 0ACE45DF57A5F981 CRC64;
MSLLQSALDF LAGPGSLGGA SGRDQSDFVG QTVELGELRL RVRRVLAEGG
FAFVYEAQDV GSGREYALKR LLSNEEEKNR AIIQEVCFMK KLSGHPNIVQ
FCSAASIGKE ESDTGQAEFL LLTELCKGQL VEFLKKMESR GPLSCDTVLK
IFYQTCRAVQ HMHRQKPPII HRDLKVENLL LSNQGTIKLC DFGSATTISH
YPDYSWSAQR RALVEEEITR NTTPMYRTPE IIDLYSNFPI GEKQDIWALG
CILYLLCFRQ HPFEDGAKLR IVNGKYSIPP HDTQYTVFHS LIRAMLQVNP
EERLSIAEVV HQLQEIAAAR NVNPKSPITE LLEQNGGYGS ATLSRGPPPP
VGPAGSGYSG GLALAEYDQP YGGFLDILRG GTERLFTNLK DTSSKVIQSV
ANYAKGDLDI SYITSRIAVM SFPAEGVESA LKNNIEDVRL FLDSKHPGHY
AVYNLSPRTY RPSRFHNRVS ECGWAARRAP HLHTLYNICR NMHAWLRQDH
KNVCVVHCMD GRAASAVAVC SFLCFCRLFS TAEAAVYMFS MKRCPPGIWP
SHKRYIEYMC DMVAEEPITP HSKPILVRAV VMTPVPLFSK QRSGCRPFCE
VYVGDERVAS TSQEYDKMRD FKIEDGKAVI PLGVTVQGDV LIVIYHARST
LGGRLQAKMA SMKMFQIQFH TGFVPRNATT VKFAKYDLDA CDIQEKYPDL
FQVNLEVEVE PRDRPSREAP PWENSSMRGL NPKILFSSRE EQQDILSKFG
KPELPRQPGS TAQYDAGAGS PEAEPTDSDS PPSSSADASR FLHTLDWQEE
KEAETGAENA SSKESESALM EDRDESEVSD EGGSPISSEG QEPRADPEPP
GLAAGLVQQD LVFEVETPAV LPEPVPQEDG VDLLGLHSEV GAGPAVPPQA
CKAPSSNTDL LSCLLGPPEA ASQGPPEDLL SEDPLLLASP APPLSVQSTP
RGGPPAAADP FGPLLPSSGN NSQPCSNPDL FGEFLNSDSV TVPPSFPSAH
SAPPPSCSAD FLHLGDLPGE PSKMTASSSN PDLLGGWAAW TETAASAVAP
TPATEGPLFS PGGQPAPCGS QASWTKSQNP DPFADLGDLS SGLQGSPAGF
PPGGFIPKTA TTPKGSSSWQ TSRPPAQGAS WPPQAKPPPK ACTQPRPNYA
SNFSVIGARE ERGVRAPSFA QKPKVSENDF EDLLSNQGFS SRSDKKGPKT
IAEMRKQDLA KDTDPLKLKL LDWIEGKERN IRALLSTLHT VLWDGESRWT
PVGMADLVAP EQVKKHYRRA VLAVHPDKAA GQPYEQHAKM IFMELNDAWS
EFENQGSRPL F
UCSC uc003gbm.6; human. [O14976-1];

Category

Human GAK ELISA Kit

Human DNAJ26 ELISA Kit;Human DNAJC26 ELISA Kit;Human cyclin G associated kinase ELISA Kit;Human cyclin-G-associated kinase ELISA Kit;Human auxilin-2 ELISA Kit;