Human CCNE1 ELISA Kit

Alternative name
Human CCNE ELISA Kit;Human pCCNE1 ELISA Kit;Human cyclin E1 ELISA Kit;Human G1/S-specific cyclin-E1 ELISA Kit;
Catalog
E010612
species
Human
GeneCCNE1
Other Species Mouse Ccne1 ELISA Kit Chicken CCNE1 ELISA Kit Rat Ccne1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CCNE1. No significant cross-reactivity or interference between human CCNE1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CCNE1 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman CCNE1 ELISA Kit allows for the in vitro quantitative determination of CCNE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCNE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCNE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCNE1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCNE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human G1/S-specific cyclin-E1 Protein information
Uniprot ID CCNE1_HUMAN
Uniprot AC P24864; A8K684; Q14091; Q8NFG1; Q92501; Q9UD21;
UniGene Hs.244723;
GeneID 898
KEGG hsa:898;
Human G1/S-specific cyclin-E1 Protein SEQUENCE
SEQUENCE 410 AA; 47077 MW; 424DF0B253B7047E CRC64;
MPRERRERDA KERDTMKEDG GAEFSARSRK RKANVTVFLQ DPDEEMAKID
RTARDQCGSQ PWDNNAVCAD PCSLIPTPDK EDDDRVYPNS TCKPRIIAPS
RGSPLPVLSW ANREEVWKIM LNKEKTYLRD QHFLEQHPLL QPKMRAILLD
WLMEVCEVYK LHRETFYLAQ DFFDRYMATQ ENVVKTLLQL IGISSLFIAA
KLEEIYPPKL HQFAYVTDGA CSGDEILTME LMIMKALKWR LSPLTIVSWL
NVYMQVAYLN DLHEVLLPQY PQQIFIQIAE LLDLCVLDVD CLEFPYGILA
ASALYHFSSS ELMQKVSGYQ WCDIENCVKW MVPFAMVIRE TGSSKLKHFR
GVADEDAHNI QTHRDSLDLL DKARAKKAML SEQNRASPLP SGLLTPPQSG
KKQSSGPEMA
UCSC uc002nsn.4; human. [P24864-1];

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Category

Human CCNE1 ELISA Kit

Human CCNE ELISA Kit;Human pCCNE1 ELISA Kit;Human cyclin E1 ELISA Kit;Human G1/S-specific cyclin-E1 ELISA Kit;