Human AKAP1 ELISA Kit

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  • Alternative name

    Human A-kinase anchor protein 149 kDa ELISA Kit;Human AKAP 149 ELISA Kit;Human Dual specificity A-kinase-anchoring protein 1 ELISA Kit;Human D-AKAP-1 ELISA Kit;Human Protein kinase A-anchoring protein 1 ELISA Kit;Human PRKA1 ELISA Kit;Human spermatid A-kinase anchor protein 84 ELISA Kit;Human S-AKAP84 ELISA Kit;Human AKAP149 ELISA Kit;Human AKAP ELISA Kit;Human AKAP121 ELISA Kit;Human AKAP84 ELISA Kit;Human D-AKAP1 ELISA Kit;Human PPP1R43 ELISA Kit;Human SAKAP84 ELISA Kit;Human TDRD17 ELISA Kit;Human A-kinase anchoring protein 1 ELISA Kit;Human A-kinase anchor protein 1, mitochondrial ELISA Kit;Human A kinase (PRKA) anchor protein 1 ELISA Kit;Human dual-specificity A-kinase anchoring protein 1 ELISA Kit;Human protein kinase A anchoring protein 1 ELISA Kit;Human protein kinase A1 ELISA Kit;Human protein phosphatase 1, regulatory subunit 43 ELISA Kit;Human testicular secretory protein Li 5 ELISA Kit;Human tudor domain containing 17 ELISA Kit;

  • Catalog
    E000106
  • species
    Human
  • GeneAKAP1
  • Standard CurveHuman AKAP1 ELISA Kit
  • Other Species Mouse Akap1 ELISA KitRat Akap1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AKAP1 ELISA Kit allows for the in vitro quantitative determination of AKAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE: This BMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY: BMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP-1 antibody and an BMP-1-HRP conjugate. The assay sample and buffer are incubated together with BMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP-1 concentration since BMP-1 from samples and BMP-1-HRP conjugate compete for the anti-BMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP-1 from the sample, fewer sites are left to bind BMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP-1 concentration in each sample is interpolated from this standard curve.
  • Human A-kinase anchor protein 1, mitochondrial Protein information
  • Uniprot ID AKAP1_HUMAN
  • Uniprot AC Q92667; A8K8Q1; D3DTZ0; Q13320; Q9BW14;
  • UniGene Hs.463506;
  • GeneID 8165
  • KEGG hsa:8165;
  • Human A-kinase anchor protein 1, mitochondrial Protein SEQUENCE
  • SEQUENCE 903 AA; 97342 MW; 0C906FAFFB0DBAF7 CRC64;

    MAIQFRSLFP LALPGMLALL GWWWFFSRKK GHVSSHDEQQ VEAGAVQLRA

    DPAIKEPLPV EDVCPKVVST PPSVTEPPEK ELSTVSKLPA EPPALLQTHP

    PCRRSESSGI LPNTTDMRLR PGTRRDDSTK LELALTGGEA KSIPLECPLS

    SPKGVLFSSK SAEVCKQDSP FSRVPRKVQP GYPVVPAEKR SSGERARETG

    GAEGTGDAVL GEKVLEEALL SREHVLELEN SKGPSLASLE GEEDKGKSSS

    SQVVGPVQEE EYVAEKLPSR FIESAHTELA KDDAAPAPPV ADAKAQDRGV

    EGELGNEESL DRNEEGLDRN EEGLDRNEES LDRNEEGLDR NEEIKRAAFQ

    IISQVISEAT EQVLATTVGK VAGRVCQASQ LQGQKEESCV PVHQKTVLGP

    DTAEPATAEA AVAPPDAGLP LPGLPAEGSP PPKTYVSCLK SLLSSPTKDS

    KPNISAHHIS LASCLALTTP SEELPDRAGI LVEDATCVTC MSDSSQSVPL

    VASPGHCSDS FSTSGLEDSC TETSSSPRDK AITPPLPEST VPFSNGVLKG

    ELSDLGAEDG WTMDAEADHS GGSDRNSMDS VDSCCSLKKT ESFQNAQAGS

    NPKKVDLIIW EIEVPKHLVG RLIGKQGRYV SFLKQTSGAK IYISTLPYTQ

    SVQICHIEGS QHHVDKALNL IGKKFKELNL TNIYAPPLPS LALPSLPMTS

    WLMLPDGITV EVIVVNQVNA GHLFVQQHTH PTFHALRSLD QQMYLCYSQP

    GIPTLPTPVE ITVICAAPGA DGAWWRAQVV ASYEETNEVE IRYVDYGGYK

    RVKVDVLRQI RSDFVTLPFQ GAEVLLDSVM PLSDDDQFSP EADAAMSEMT

    GNTALLAQVT SYSPTGLPLI QLWSVVGDEV VLINRSLVER GLAQWVDSYY

    TSL

  • UCSC uc002iux.4; human. [Q92667-1];


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