Human CDK8 ELISA Kit

Alternative name
Human cell division protein kinase 8 ELISA Kit;Human mediator complex subunit CDK8 ELISA Kit;Human mediator of RNA polymerase II transcription subunit CDK8 ELISA Kit;Human protein kinase K35 ELISA Kit;Human K35 ELISA Kit;Human cyclin dependent kinase 8 ELISA Kit;Human cyclin-dependent kinase 8 ELISA Kit;Human CDK8 protein kinase ELISA Kit;
Catalog
E010588
species
Human
GeneCDK8
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 8 (CDK8). No significant cross-reactivity or interference between Cyclin Dependent Kinase 8 (CDK8) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.127ng/mL.
Intended UseHuman CDK8 ELISA Kit allows for the in vitro quantitative determination of CDK8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 8 (CDK8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 8 (CDK8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 8 (CDK8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 8 (CDK8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cyclin-dependent kinase 8 Protein information
Uniprot ID CDK8_HUMAN
Uniprot AC P49336; Q5VUF3; Q6ISB5;
UniGene Hs.382306; Hs.695166;
GeneID 1024
KEGG hsa:1024;
Human Cyclin-dependent kinase 8 Protein SEQUENCE
SEQUENCE 464 AA; 53284 MW; 00CBAB6CE354605F CRC64;
MDYDFKVKLS SERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDKDYA
LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA
EHDLWHIIKF HRASKANKKP VQLPRGMVKS LLYQILDGIH YLHANWVLHR
DLKPANILVM GEGPERGRVK IADMGFARLF NSPLKPLADL DPVVVTFWYR
APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD
QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH
KVKPDSKAFH LLQKLLTMDP IKRITSEQAM QDPYFLEDPL PTSDVFAGCQ
IPYPKREFLT EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDSSHTQGPP
LKKVRVVPPT TTSGGLIMTS DYQRSNPHAA YPNPGPSTSQ PQSSMGYSAT
SQQPPQYSHQ THRY
UCSC uc001uqr.2; human. [P49336-1];

Category

Human CDK8 ELISA Kit