Human CDK6 ELISA Kit

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  • Alternative name

    Human cell division protein kinase 6 ELISA Kit;Human serine/threonine-protein kinase PLSTIRE ELISA Kit;Human CDKN6 ELISA Kit;Human MCPH12 ELISA Kit;Human PLSTIRE ELISA Kit;Human cyclin dependent kinase 6 ELISA Kit;Human cyclin-dependent kinase 6 ELISA Kit;

  • Catalog
    E010580
  • species
    Human
  • GeneCDK6
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 6 (CDK6). No significant cross-reactivity or interference between Cyclin Dependent Kinase 6 (CDK6) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman CDK6 ELISA Kit allows for the in vitro quantitative determination of CDK6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 6 (CDK6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 6 (CDK6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 6 (CDK6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 6 (CDK6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cyclin-dependent kinase 6 Protein information
  • Uniprot ID CDK6_HUMAN
  • Uniprot AC Q00534; A4D1G0;
  • UniGene Hs.119882;
  • GeneID 1021
  • KEGG hsa:1021;
  • Human Cyclin-dependent kinase 6 Protein SEQUENCE
  • SEQUENCE 326 AA; 36938 MW; 571733EE6BE7FD4A CRC64;

    MEKDGLCRAD QQYECVAEIG EGAYGKVFKA RDLKNGGRFV ALKRVRVQTG

    EEGMPLSTIR EVAVLRHLET FEHPNVVRLF DVCTVSRTDR ETKLTLVFEH

    VDQDLTTYLD KVPEPGVPTE TIKDMMFQLL RGLDFLHSHR VVHRDLKPQN

    ILVTSSGQIK LADFGLARIY SFQMALTSVV VTLWYRAPEV LLQSSYATPV

    DLWSVGCIFA EMFRRKPLFR GSSDVDQLGK ILDVIGLPGE EDWPRDVALP

    RQAFHSKSAQ PIEKFVTDID ELGKDLLLKC LTFNPAKRIS AYSALSHPYF

    QDLERCKENL DSHLPPSQNT SELNTA

  • UCSC uc010lez.4; human.;



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