Human CDK2 ELISA Kit

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  • Alternative name

    Human cell division protein kinase 2 ELISA Kit;Human p33 protein kinase ELISA Kit;Human CDKN2 ELISA Kit;Human p33(CDK2) ELISA Kit;Human cyclin dependent kinase 2 ELISA Kit;Human cyclin-dependent kinase 2 ELISA Kit;Human cdc2-related protein kinase ELISA Kit;

  • Catalog
    E010560
  • species
    Human
  • GeneCDK2
  • Standard CurveHuman CDK2 ELISA Kit
  • Other Species Mouse Cdk2 ELISA KitBovine CDK2 ELISA KitHamster CDK2 ELISA KitRat Cdk2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 2 (CDK2). No significant cross-reactivity or interference between Cyclin Dependent Kinase 2 (CDK2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman CDK2 ELISA Kit allows for the in vitro quantitative determination of CDK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 2 (CDK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 2 (CDK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 2 (CDK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 2 (CDK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cyclin-dependent kinase 2 Protein information
  • Uniprot ID CDK2_HUMAN
  • Uniprot AC P24941; A8K7C6; O75100;
  • UniGene Hs.19192; Hs.689624;
  • GeneID 1017
  • KEGG hsa:1017;
  • Human Cyclin-dependent kinase 2 Protein SEQUENCE
  • SEQUENCE 298 AA; 33930 MW; F90A0F4E70910B51 CRC64;

    MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR

    EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP

    LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR

    AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR

    ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK

    VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL

  • UCSC uc001sit.5; human. [P24941-1];



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