Intended UseSheep Estrone ELISA Kit allows for the in vitro quantitative determination of Estrone , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole kit at 4℃
Product Description specificalSheep Estrone ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to Estrone, During the reaction,Estrone in the sample or standard competes with a fixed amount of biotin-labeled Estrone for sites on a pre-coated Monoclonal antibody specific to Estrone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Estrone in the samples is then determined by comparing the O.D. of the samples to the standard curve.