androstenedioneprostaglandin e2galectin-3hyaluronic acidasprosin

Home Human CCND3 ELISA Kit

Category

Human CCND3 ELISA Kit

Contact Us
  • Alternative name

    Human cyclin D3 ELISA Kit;Human G1/S-specific cyclin-D3 ELISA Kit;Human D3-type cyclin ELISA Kit;
  • Catalog
    E010542
  • species
    Human
  • GeneCCND3
  • Standard CurveHuman CCND3 ELISA Kit
  • Other Species Mouse Ccnd3 ELISA KitBovine CCND3 ELISA KitRat Ccnd3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CCND3. No significant cross-reactivity or interference between CCND3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCND3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CCND3 ELISA Kit allows for the in vitro quantitative determination of CCND3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Intended Uses: This CCND3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCND3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CCND3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCND3 antibody and an CCND3-HRP conjugate. The assay sample and buffer are incubated together with CCND3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCND3 concentration since CCND3 from samples and CCND3-HRP conjugate compete for the anti-CCND3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCND3 from the sample, fewer sites are left to bind CCND3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND3 concentration in each sample is interpolated from this standard curve.
  • Human G1/S-specific cyclin-D3 Protein information
  • Uniprot ID CCND3_HUMAN
  • Uniprot AC P30281; B2RD63; B3KQ22; E9PAS4; E9PB36; Q5T8J0; Q6FG62; Q96F49;
  • UniGene Hs.534307;
  • GeneID 896
  • KEGG hsa:896;
  • Human G1/S-specific cyclin-D3 Protein SEQUENCE

  • SEQUENCE 292 AA; 32520 MW; 16E7B1604FEB0029 CRC64;

    MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR

    EIKPHMRKML AYWMLEVCEE QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ

    LLGAVCMLLA SKLRETTPLT IEKLCIYTDH AVSPRQLRDW EVLVLGKLKW

    DLAAVIAHDF LAFILHRLSL PRDRQALVKK HAQTFLALCA TDYTFAMYPP

    SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA

    LRESLREASQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL

  • UCSC uc003orn.4; human. [P30281-1];


Related products with Human CCND3 ELISA Kit

Inquiry About Human CCND3 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!