Human CCND3 ELISA Kit

Alternative name
Human cyclin D3 ELISA Kit;Human G1/S-specific cyclin-D3 ELISA Kit;Human D3-type cyclin ELISA Kit;
Catalog
E010542
species
Human
GeneCCND3
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCND3. No significant cross-reactivity or interference between CCND3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCND3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CCND3 ELISA Kit allows for the in vitro quantitative determination of CCND3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CCND3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCND3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CCND3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCND3 antibody and an CCND3-HRP conjugate. The assay sample and buffer are incubated together with CCND3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCND3 concentration since CCND3 from samples and CCND3-HRP conjugate compete for the anti-CCND3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCND3 from the sample, fewer sites are left to bind CCND3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND3 concentration in each sample is interpolated from this standard curve.
Human G1/S-specific cyclin-D3 Protein information
Uniprot ID CCND3_HUMAN
Uniprot AC P30281; B2RD63; B3KQ22; E9PAS4; E9PB36; Q5T8J0; Q6FG62; Q96F49;
UniGene Hs.534307;
GeneID 896
KEGG hsa:896;
Human G1/S-specific cyclin-D3 Protein SEQUENCE
SEQUENCE 292 AA; 32520 MW; 16E7B1604FEB0029 CRC64;
MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQR
EIKPHMRKML AYWMLEVCEE QRCEEEVFPL AMNYLDRYLS CVPTRKAQLQ
LLGAVCMLLA SKLRETTPLT IEKLCIYTDH AVSPRQLRDW EVLVLGKLKW
DLAAVIAHDF LAFILHRLSL PRDRQALVKK HAQTFLALCA TDYTFAMYPP
SMIATGSIGA AVQGLGACSM SGDELTELLA GITGTEVDCL RACQEQIEAA
LRESLREASQ TSSSPAPKAP RGSSSQGPSQ TSTPTDVTAI HL
UCSC uc003orn.4; human. [P30281-1];

Human CCND3 ELISA Kit

Human cyclin D3 ELISA Kit;Human G1/S-specific cyclin-D3 ELISA Kit;Human D3-type cyclin ELISA Kit;

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