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Alternative name
Leukotriene B4 ELISA Kit
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Catalog
E105067
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species
Hamster
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GeneLT-B4
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Standard Curve
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Other Species
Human LT-B4 ELISA KitMouse LT-B4 ELISA KitBovine LT-B4 ELISA KitCanine LT-B4 ELISA KitChicken LT-B4 ELISA KitGeneral LT-B4 ELISA KitPorcine LT-B4 ELISA KitRabbit LT-B4 ELISA KitRat LT-B4 ELISA KitCamel LT-B4 ELISA KitGoat LT-B4 ELISA KitGuinea Pig LT-B4 ELISA KitHorse LT-B4 ELISA KitMonkey LT-B4 ELISA KitSheep LT-B4 ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity9.375pg/ml
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Intended UseHamster LT-B4 ELISA Kit allows for the in vitro quantitative determination of LT-B4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHamster LT-B4 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to LT-B4 , During the reaction,LT-B4 in the sample or standard competes with a fixed amount of biotin-labeled LT-B4 for sites on a pre-coated Monoclonal antibody specific to LT-B4 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LT-B4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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