Human CYB561 ELISA Kit

Alternative name
Human cytochrome b-561 ELISA Kit;Human CYB561A1 ELISA Kit;Human FRRS2 ELISA Kit;Human cytochrome b561 ELISA Kit;Human cytochrome b561 family, member A1 ELISA Kit;Human ferric-chelate reductase 2 ELISA Kit;
Catalog
E010477
species
Human
GeneCYB561
Other Species Mouse Cyb561 ELISA Kit Bovine CYB561 ELISA Kit Porcine CYB561 ELISA Kit Sheep CYB561 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CYB561. No significant cross-reactivity or interference between Human CYB561 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CYB561 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman CYB561 ELISA Kit allows for the in vitro quantitative determination of CYB561 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CYB561 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CYB561. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CYB561 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYB561, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CYB561. You can calculate the concentration of Human CYB561 in the samples by comparing the OD of the samples to the standard curve.
Human Cytochrome b561 Protein information
Uniprot ID CY561_HUMAN
Uniprot AC P49447; B2RE96; B7Z775; D3DU11; Q5BJG9; Q9BU05; Q9BWR9;
UniGene Hs.355264;
GeneID 1534
KEGG hsa:1534;
Human Cytochrome b561 Protein SEQUENCE
SEQUENCE 251 AA; 27559 MW; 8EE53AE4D2569B77 CRC64;
MEGGAAAATP TALPYYVAFS QLLGLTLVAM TGAWLGLYRG GIAWESDLQF
NAHPLCMVIG LIFLQGNALL VYRVFRNEAK RTTKVLHGLL HIFALVIALV
GLVAVFDYHR KKGYADLYSL HSWCGILVFV LYFVQWLVGF SFFLFPGASF
SLRSRYRPQH IFFGATIFLL SVGTALLGLK EALLFNLGGK YSAFEPEGVL
ANVLGLLLAC FGGAVLYILT RADWKRPSQA EEQALSMDFK TLTEGDSPGS
Q
UCSC uc002jap.4; human. [P49447-1];

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Human CYB561 ELISA Kit

Human cytochrome b-561 ELISA Kit;Human CYB561A1 ELISA Kit;Human FRRS2 ELISA Kit;Human cytochrome b561 ELISA Kit;Human cytochrome b561 family, member A1 ELISA Kit;Human ferric-chelate reductase 2 ELISA Kit;