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Human PF4 ELISA Kit

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  • Alternative name

    Human PF-4 ELISA Kit;Human C-X-C motif chemokine 4 ELISA Kit;Human iroplact ELISA Kit;Human oncostatin-A ELISA Kit;Human CXCL4 ELISA Kit;Human SCYB4 ELISA Kit;Human platelet factor 4 ELISA Kit;Human chemokine (C-X-C motif) ligand 4 ELISA Kit;
  • Catalog
    E010463
  • species
    Human
  • GenePF4
  • Standard CurveHuman PF4 ELISA Kit
  • Other Species Human H-PF4 ELISA KitMouse Pf4 ELISA KitMouse H-PF4 ELISA KitMouse PF4/CXCL4 ELISA KitMouse CXCL4/PF4 ELISA KitBovine PF4 ELISA KitPorcine PF4 ELISA KitRabbit PF4 ELISA KitRat Pf4 ELISA KitSheep PF4 ELISA Kit
  • SpecificityNatural and recombinant human CXCL4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human CXCL4 in cell culture supernates, serum and plas
  • Intended UseHuman PF4 ELISA Kit allows for the in vitro quantitative determination of PF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: Human CXCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL4 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: E32-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL4 amount of sample captured in plate. Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
  • Human Platelet factor 4, short form Protein information
  • Uniprot ID PLF4_HUMAN
  • Uniprot AC P02776; Q53X61; Q9UC64; Q9UC65;
  • UniGene Hs.81564;
  • GeneID 5196
  • KEGG hsa:5196;
  • Human Platelet factor 4, short form Protein SEQUENCE

  • SEQUENCE 101 AA; 10845 MW; E96C2EFE9B944D85 CRC64;

    MSSAAGFCAS RPGLLFLGLL LLPLVVAFAS AEAEEDGDLQ CLCVKTTSQV

    RPRHITSLEV IKAGPHCPTA QLIATLKNGR KICLDLQAPL YKKIIKKLLE

    S

  • UCSC uc003hhi.4; human.;


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