Rat 2,3-BPG ELISA Kit

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  • Alternative name

    2 ELISA Kit,3-Bisphosphoglycerate ELISA Kit,2 ELISA Kit,3-BPG ELISA Kit,2 ELISA Kit,3-Bisphosphoglyceric acid ELISA Kit,2 ELISA Kit,3-diphosphoglycerate ELISA Kit

  • Catalog
    E104533
  • species
    Rat
  • Gene2,3-BPG
  • Standard CurveRat 2,3-BPG ELISA Kit
  • Other Species Bovine 2,3-BPG ELISA KitCanine 2,3-BPG ELISA KitChicken 2,3-BPG ELISA KitHuman 2,3-BPG ELISA KitGeneral 2,3-BPG ELISA KitMouse 2,3-BPG ELISA KitPorcine 2,3-BPG ELISA KitRabbit 2,3-BPG ELISA KitCamel 2,3-BPG ELISA KitGoat 2,3-BPG ELISA KitGuinea Pig 2,3-BPG ELISA KitHamster 2,3-BPG ELISA KitHorse 2,3-BPG ELISA KitMonkey 2,3-BPG ELISA KitSheep 2,3-BPG ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity7.5 nmol/mL
  • Intended UseRat 2,3-BPG ELISA Kit allows for the in vitro quantitative determination of 2,3-BPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Rat 2,3-BPG ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 2,3-BPG, During the reaction,2,3-BPG in the sample or standard competes with a fixed amount of biotin-labeled 2,3-BPG for sites on a pre-coated Monoclonal antibody specific to 2,3-BPG. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 2,3-BPG in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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