Porcine DAG ELISA Kit

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  • Alternative name

    Diacylglycerol ELISA Kit,DAG ELISA Kit,Diglyceride ELISA Kit

  • Catalog
  • species
  • GeneDAG
  • Standard CurvePorcine DAG ELISA Kit
  • Other Species Human DAG ELISA KitMouse DAG ELISA KitBovine DAG ELISA KitCanine DAG ELISA KitChicken DAG ELISA KitGeneral DAG ELISA KitRabbit DAG ELISA KitRat DAG ELISA KitCamel DAG ELISA KitGoat DAG ELISA KitGuinea Pig DAG ELISA KitHamster DAG ELISA KitHorse DAG ELISA KitMonkey DAG ELISA KitSheep DAG ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.156ng/ml
  • Intended UsePorcine DAG ELISA Kit allows for the in vitro quantitative determination of DAG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    Porcine DAG ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to DAG, During the reaction,DAG in the sample or standard competes with a fixed amount of biotin-labeled DAG for sites on a pre-coated Monoclonal antibody specific to DAG. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DAG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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