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Alternative name
Vanillylmandelic acid ELISA Kit,VMA ELISA Kit
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Catalog
E104309
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species
Porcine
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GeneVMA
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Standard Curve
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Other Species
Human VMA ELISA KitMouse VMA ELISA KitBovine VMA ELISA KitCanine VMA ELISA KitChicken VMA ELISA KitGeneral VMA ELISA KitRabbit VMA ELISA KitRat VMA ELISA KitCamel VMA ELISA KitGoat VMA ELISA KitGuinea Pig VMA ELISA KitHamster VMA ELISA KitHorse VMA ELISA KitMonkey VMA ELISA KitSheep VMA ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity2.2ng/ml
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Intended UsePorcine VMA ELISA Kit allows for the in vitro quantitative determination of VMA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalPorcine VMA ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to VMA, During the reaction,VMA in the sample or standard competes with a fixed amount of biotin-labeled VMA for sites on a pre-coated Monoclonal antibody specific to VMA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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