Porcine 12HETE ELISA Kit

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  • Alternative name

    12-Hydroxyeicosatetraenoic acid ELISA Kit,12HETE ELISA Kit

  • Catalog
    E104300
  • species
    Porcine
  • Gene12HETE
  • Standard CurvePorcine 12HETE ELISA Kit
  • Other Species Bovine 12HETE ELISA KitCanine 12HETE ELISA KitChicken 12HETE ELISA KitHuman 12HETE ELISA KitGeneral 12HETE ELISA KitMouse 12HETE ELISA KitRabbit 12HETE ELISA KitRat 12HETE ELISA KitCamel 12HETE ELISA KitGoat 12HETE ELISA KitGuinea Pig 12HETE ELISA KitHamster 12HETE ELISA KitHorse 12HETE ELISA KitMonkey 12HETE ELISA KitSheep 12HETE ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.056ng/ml
  • Intended UsePorcine 12HETE ELISA Kit allows for the in vitro quantitative determination of 12HETE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Porcine 12HETE ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 12HETE, During the reaction,12HETE in the sample or standard competes with a fixed amount of biotin-labeled 12HETE for sites on a pre-coated Monoclonal antibody specific to 12HETE. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12HETE in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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