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Alternative name
Serotonin ELISA Kit,5HT ELISA Kit,5-hydroxytryptamine ELISA Kit
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Catalog
E104279
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species
Porcine
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Gene5HT
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Standard Curve
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Other Species
Human 5HT ELISA KitHuman 5HT/ST ELISA KitMouse 5HT ELISA KitBovine 5HT ELISA KitCanine 5HT ELISA KitChicken 5HT ELISA KitGeneral 5HT ELISA KitRabbit 5HT ELISA KitRat 5HT ELISA KitCamel 5HT ELISA KitGoat 5HT ELISA KitGuinea Pig 5HT ELISA KitHamster 5HT ELISA KitHorse 5HT ELISA KitMonkey 5HT ELISA KitSheep 5HT ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity0.23ng/ml
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Intended UsePorcine 5HT ELISA Kit allows for the in vitro quantitative determination of 5HT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalPorcine 5HT ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 5HT, During the reaction,5HT in the sample or standard competes with a fixed amount of biotin-labeled 5HT for sites on a pre-coated Monoclonal antibody specific to 5HT. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5HT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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