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Alternative name
Ciclosporin ELISA Kit,CsA ELISA Kit,Cyclosporine A ELISA Kit
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Catalog
E104271
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species
Porcine
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GeneCsA
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Standard Curve
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Other Species
Human CsA ELISA KitMouse CsA ELISA KitBovine CsA ELISA KitCanine CsA ELISA KitChicken CsA ELISA KitGeneral CsA ELISA KitRabbit CsA ELISA KitRat CsA ELISA KitCamel CsA ELISA KitGoat CsA ELISA KitGuinea Pig CsA ELISA KitHamster CsA ELISA KitHorse CsA ELISA KitMonkey CsA ELISA KitSheep CsA ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity1.2ng/mL
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Intended UsePorcine CsA ELISA Kit allows for the in vitro quantitative determination of CsA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalPorcine CsA ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to CsA, During the reaction,CsA in the sample or standard competes with a fixed amount of biotin-labeled CsA for sites on a pre-coated Monoclonal antibody specific to CsA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CsA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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