-
Alternative name
Flavin mononucleotide ELISA Kit
-
Catalog
E104202
-
GeneFMN
-
Standard Curve
-
Other Species
Bovine FMN ELISA KitCanine FMN ELISA KitChicken FMN ELISA KitHuman FMN ELISA KitMouse FMN ELISA KitPorcine FMN ELISA KitRabbit FMN ELISA KitRat FMN ELISA KitCamel FMN ELISA KitGoat FMN ELISA KitGuinea Pig FMN ELISA KitHamster FMN ELISA KitHorse FMN ELISA KitMonkey FMN ELISA KitSheep FMN ELISA Kit
-
SpecificityNo cross-reaction with other factors
-
Sensitivity9.375pg/ml
-
Intended UseGeneral FMN ELISA Kit allows for the in vitro quantitative determination of FMN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole kit at 4℃
-
Product Description
specificalGeneral FMN ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to FMN , During the reaction,FMN in the sample or standard competes with a fixed amount of biotin-labeled FMN for sites on a pre-coated Monoclonal antibody specific to FMN . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FMN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-