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General VB1 ELISA Kit

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  • Alternative name

    Vitamin B1 ELISA Kit,VB1 ELISA Kit,Thiamine ELISA Kit,thiamin ELISA Kit
  • Catalog
    E104178
  • GeneVB1
  • Standard CurveGeneral VB1 ELISA Kit
  • Other Species Mouse VB1 ELISA KitBovine VB1 ELISA KitCanine VB1 ELISA KitChicken VB1 ELISA KitHuman VB1 ELISA KitPorcine VB1 ELISA KitRabbit VB1 ELISA KitRat VB1 ELISA KitCamel VB1 ELISA KitGoat VB1 ELISA KitGuinea Pig VB1 ELISA KitHamster VB1 ELISA KitHorse VB1 ELISA KitMonkey VB1 ELISA KitSheep VB1 ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity1ng/ml
  • Intended UseGeneral VB1 ELISA Kit allows for the in vitro quantitative determination of VB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical    General VB1 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to VB1, During the reaction,VB1 in the sample or standard competes with a fixed amount of biotin-labeled VB1 for sites on a pre-coated Monoclonal antibody specific to VB1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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