General VA ELISA Kit

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  • Alternative name

    Vitamin A ELISA Kit

  • Catalog
  • GeneVA
  • Standard CurveGeneral VA ELISA Kit
  • Other Species Mouse VA ELISA KitBovine VA ELISA KitCanine VA ELISA KitChicken VA ELISA KitHuman VA ELISA KitPorcine VA ELISA KitRabbit VA ELISA KitRat VA ELISA KitCamel VA ELISA KitGoat VA ELISA KitGuinea Pig VA ELISA KitHamster VA ELISA KitHorse VA ELISA KitMonkey VA ELISA KitSheep VA ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.094ng/ml
  • Intended UseGeneral VA ELISA Kit allows for the in vitro quantitative determination of VA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    General VA ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to VA , During the reaction,VA in the sample or standard competes with a fixed amount of biotin-labeled VA for sites on a pre-coated Monoclonal antibody specific to VA . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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