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Alternative name
Prostaglandin E1 ELISA Kit
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Catalog
E104044
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species
Chicken
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GenePGE1
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Standard Curve
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Other Species
Human PGE1 ELISA KitHuman PGE1 ELISA KitMouse PGE1 ELISA KitBovine PGE1 ELISA KitCanine PGE1 ELISA KitGeneral PGE1 ELISA KitPorcine PGE1 ELISA KitRabbit PGE1 ELISA KitRat PGE1 ELISA KitCamel PGE1 ELISA KitGoat PGE1 ELISA KitGuinea Pig PGE1 ELISA KitHamster PGE1 ELISA KitHorse PGE1 ELISA KitMonkey PGE1 ELISA KitSheep PGE1 ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity46.875pg/ml
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Intended UseChicken PGE1 ELISA Kit allows for the in vitro quantitative determination of PGE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalChicken PGE1 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to PGE1 , During the reaction,PGE1 in the sample or standard competes with a fixed amount of biotin-labeled PGE1 for sites on a pre-coated Monoclonal antibody specific to PGE1 . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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