Chicken SPH ELISA Kit

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  • Alternative name

    Sphingosine ELISA Kit,SPH ELISA Kit

  • Catalog
  • species
  • GeneSPH
  • Standard CurveChicken SPH ELISA Kit
  • Other Species Human SM/SPH ELISA KitHuman SPH ELISA KitMouse SPH ELISA KitBovine SPH ELISA KitCanine SPH ELISA KitGeneral SPH ELISA KitPorcine SPH ELISA KitRabbit SPH ELISA KitRat SPH ELISA KitCamel SPH ELISA KitGoat SPH ELISA KitGuinea Pig SPH ELISA KitHamster SPH ELISA KitHorse SPH ELISA KitMonkey SPH ELISA KitSheep SPH ELISA Kit
  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.1ng/ml
  • Intended UseChicken SPH ELISA Kit allows for the in vitro quantitative determination of SPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    Chicken SPH ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to SPH, During the reaction,SPH in the sample or standard competes with a fixed amount of biotin-labeled SPH for sites on a pre-coated Monoclonal antibody specific to SPH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SPH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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