Chicken MN ELISA Kit

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  • Alternative name

    Mannose ELISA Kit,MN ELISA Kit

  • Catalog
    E104004
  • species
    Chicken
  • GeneMN
  • Standard CurveChicken MN ELISA Kit
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  • SpecificityNo cross-reaction with other factors
  • Sensitivity0.23ng/ml
  • Intended UseChicken MN ELISA Kit allows for the in vitro quantitative determination of MN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole kit at 4℃
  • Product Description
    specifical
    Chicken MN ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to MN, During the reaction,MN in the sample or standard competes with a fixed amount of biotin-labeled MN for sites on a pre-coated Monoclonal antibody specific to MN. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MN in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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