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Alternative name
Inositol trisphosphate ELISA Kit,IP3 ELISA Kit,Inositol 1 ELISA Kit,4 ELISA Kit,5-trisphosphate ELISA Kit
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Catalog
E103898
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species
Canine
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GeneIP3
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Standard Curve
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Other Species
Human IP3 ELISA KitMouse IP3 ELISA KitBovine IP3 ELISA KitChicken IP3 ELISA KitGeneral IP3 ELISA KitPorcine IP3 ELISA KitRabbit IP3 ELISA KitRat IP3 ELISA KitCamel IP3 ELISA KitGoat IP3 ELISA KitGuinea Pig IP3 ELISA KitHamster IP3 ELISA KitHorse IP3 ELISA KitMonkey IP3 ELISA KitSheep IP3 ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity0.156ng/ml
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Intended UseCanine IP3 ELISA Kit allows for the in vitro quantitative determination of IP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalCanine IP3 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to IP3, During the reaction,IP3 in the sample or standard competes with a fixed amount of biotin-labeled IP3 for sites on a pre-coated Monoclonal antibody specific to IP3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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