Human CUL4A ELISA Kit

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  • Alternative name

    Human CUL-4A ELISA Kit;Human cullin 4A ELISA Kit;Human cullin-4A ELISA Kit;

  • Catalog
    E010384
  • species
    Human
  • GeneCUL4A
  • Standard CurveHuman CUL4A ELISA Kit
  • Other Species Mouse Cul4a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CUL4A. No significant cross-reactivity or interference between CUL4A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CUL4A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CUL4A ELISA Kit allows for the in vitro quantitative determination of CUL4A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CUL4A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CUL4A antibody and an CUL4A-HRP conjugate. The assay sample and buffer are incubated together with CUL4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CUL4A concentration since CUL4A from samples and CUL4A-HRP conjugate compete for the anti-CUL4A antibody binding site. Since the number of sites is limited, as more sites are occupied by CUL4A from the sample, fewer sites are left to bind CUL4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CUL4A concentration in each sample is interpolated from this standard curve.
  • Human Cullin-4A Protein information
  • Uniprot ID CUL4A_HUMAN
  • Uniprot AC Q13619; A2A2W2; O75834; Q589T6; Q5TC62; Q6UP08; Q9UP17;
  • UniGene Hs.339735;
  • GeneID 8451
  • KEGG hsa:8451;
  • Human Cullin-4A Protein SEQUENCE
  • SEQUENCE 759 AA; 87680 MW; 3C4C6A1BBD94D51B CRC64;

    MADEAPRKGS FSALVGRTNG LTKPAALAAA PAKPGGAGGS KKLVIKNFRD

    RPRLPDNYTQ DTWRKLHEAV RAVQSSTSIR YNLEELYQAV ENLCSHKVSP

    MLYKQLRQAC EDHVQAQILP FREDSLDSVL FLKKINTCWQ DHCRQMIMIR

    SIFLFLDRTY VLQNSTLPSI WDMGLELFRT HIISDKMVQS KTIDGILLLI

    ERERSGEAVD RSLLRSLLGM LSDLQVYKDS FELKFLEETN CLYAAEGQRL

    MQEREVPEYL NHVSKRLEEE GDRVITYLDH STQKPLIACV EKQLLGEHLT

    AILQKGLDHL LDENRVPDLA QMYQLFSRVR GGQQALLQHW SEYIKTFGTA

    IVINPEKDKD MVQDLLDFKD KVDHVIEVCF QKNERFVNLM KESFETFINK

    RPNKPAELIA KHVDSKLRAG NKEATDEELE RTLDKIMILF RFIHGKDVFE

    AFYKKDLAKR LLVGKSASVD AEKSMLSKLK HECGAAFTSK LEGMFKDMEL

    SKDIMVHFKQ HMQNQSDSGP IDLTVNILTM GYWPTYTPME VHLTPEMIKL

    QEVFKAFYLG KHSGRKLQWQ TTLGHAVLKA EFKEGKKEFQ VSLFQTLVLL

    MFNEGDGFSF EEIKMATGIE DSELRRTLQS LACGKARVLI KSPKGKEVED

    GDKFIFNGEF KHKLFRIKIN QIQMKETVEE QVSTTERVFQ DRQYQIDAAI

    VRIMKMRKTL GHNLLVSELY NQLKFPVKPG DLKKRIESLI DRDYMERDKD

    NPNQYHYVA

  • UCSC uc021rmu.2; human. [Q13619-1];


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