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Alternative name
17-Hydroxyprogesterone ELISA Kit,17OHP ELISA Kit,17-OH progesterone ELISA Kit
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Catalog
E103826
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species
Canine
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Gene17OHP
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Standard Curve
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Other Species
Bovine 17OHP ELISA KitChicken 17OHP ELISA KitHuman 17OHP ELISA KitGeneral 17OHP ELISA KitMouse 17OHP ELISA KitPorcine 17OHP ELISA KitRabbit 17OHP ELISA KitRat 17OHP ELISA KitCamel 17OHP ELISA KitGoat 17OHP ELISA KitGuinea Pig 17OHP ELISA KitHamster 17OHP ELISA KitHorse 17OHP ELISA KitMonkey 17OHP ELISA KitSheep 17OHP ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity0.12ng/ml
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Intended UseCanine 17OHP ELISA Kit allows for the in vitro quantitative determination of 17OHP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalCanine 17OHP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to 17OHP, During the reaction,17OHP in the sample or standard competes with a fixed amount of biotin-labeled 17OHP for sites on a pre-coated Monoclonal antibody specific to 17OHP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 17OHP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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