Human CUL3 ELISA Kit

Alternative name
Human CUL-3 ELISA Kit;Human KIAA0617 ELISA Kit;Human PHA2E ELISA Kit;Human cullin 3 ELISA Kit;Human cullin-3 ELISA Kit;
Catalog
E010382
species
Human
GeneCUL3
Standard Curve
Other Species Mouse Cul3 ELISA Kit Rat Cul3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CUL3. No significant cross-reactivity or interference between CUL3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CUL3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CUL3 ELISA Kit allows for the in vitro quantitative determination of CUL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CUL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CUL3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CUL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CUL3 antibody and an CUL3-HRP conjugate. The assay sample and buffer are incubated together with CUL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CUL3 concentration since CUL3 from samples and CUL3-HRP conjugate compete for the anti-CUL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CUL3 from the sample, fewer sites are left to bind CUL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CUL3 concentration in each sample is interpolated from this standard curve.
Human Cullin-3 Protein information
Uniprot ID CUL3_HUMAN
Uniprot AC Q13618; A8K536; B8ZZC3; O75415; Q569L3; Q9UBI8; Q9UET7;
UniGene Hs.372286;
GeneID 8452
KEGG hsa:8452;
Human Cullin-3 Protein SEQUENCE
SEQUENCE 768 AA; 88930 MW; A1A02022480BF099 CRC64;
MSNLSKGTGS RKDTKMRIRA FPMTMDEKYV NSIWDLLKNA IQEIQRKNNS
GLSFEELYRN AYTMVLHKHG EKLYTGLREV VTEHLINKVR EDVLNSLNNN
FLQTLNQAWN DHQTAMVMIR DILMYMDRVY VQQNNVENVY NLGLIIFRDQ
VVRYGCIRDH LRQTLLDMIA RERKGEVVDR GAIRNACQML MILGLEGRSV
YEEDFEAPFL EMSAEFFQME SQKFLAENSA SVYIKKVEAR INEEIERVMH
CLDKSTEEPI VKVVERELIS KHMKTIVEME NSGLVHMLKN GKTEDLGCMY
KLFSRVPNGL KTMCECMSSY LREQGKALVS EEGEGKNPVD YIQGLLDLKS
RFDRFLLESF NNDRLFKQTI AGDFEYFLNL NSRSPEYLSL FIDDKLKKGV
KGLTEQEVET ILDKAMVLFR FMQEKDVFER YYKQHLARRL LTNKSVSDDS
EKNMISKLKT ECGCQFTSKL EGMFRDMSIS NTTMDEFRQH LQATGVSLGG
VDLTVRVLTT GYWPTQSATP KCNIPPAPRH AFEIFRRFYL AKHSGRQLTL
QHHMGSADLN ATFYGPVKKE DGSEVGVGGA QVTGSNTRKH ILQVSTFQMT
ILMLFNNREK YTFEEIQQET DIPERELVRA LQSLACGKPT QRVLTKEPKS
KEIENGHIFT VNDQFTSKLH RVKIQTVAAK QGESDPERKE TRQKVDDDRK
HEIEAAIVRI MKSRKKMQHN VLVAEVTQQL KARFLPSPVV IKKRIEGLIE
REYLARTPED RKVYTYVA
UCSC uc002vny.4; human. [Q13618-1];

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anti-CUL3 antibody

Category

Human CUL3 ELISA Kit

Human CUL-3 ELISA Kit;Human KIAA0617 ELISA Kit;Human PHA2E ELISA Kit;Human cullin 3 ELISA Kit;Human cullin-3 ELISA Kit;