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Alternative name
Tetrahydrobiopterin ELISA Kit,THB ELISA Kit,BH4 ELISA Kit
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Catalog
E103787
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species
Bovine
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GeneTHB
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Standard Curve
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Other Species
Canine THB ELISA KitChicken THB ELISA KitHuman THB ELISA KitGeneral THB ELISA KitMouse THB ELISA KitPorcine THB ELISA KitRabbit THB ELISA KitRat THB ELISA KitCamel THB ELISA KitGoat THB ELISA KitGuinea Pig THB ELISA KitHamster THB ELISA KitHorse THB ELISA KitMonkey THB ELISA KitSheep THB ELISA Kit
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SpecificityNo cross-reaction with other factors
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Sensitivity0.051 uIU/mL
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Intended UseBovine THB ELISA Kit allows for the in vitro quantitative determination of THB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalBovine THB ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to THB, During the reaction,THB in the sample or standard competes with a fixed amount of biotin-labeled THB for sites on a pre-coated Monoclonal antibody specific to THB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of THB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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