Human CSF3R ELISA Kit

Alternative name
Human G-CSF receptor ELISA Kit;Human G-CSF-R ELISA Kit;Human GCSFR ELISA Kit;Human CD114 ELISA Kit;Human SCN7 ELISA Kit;Human colony stimulating factor 3 receptor ELISA Kit;Human granulocyte colony-stimulating factor receptor ELISA Kit;Human CD114 antigen ELISA Kit;Human colony stimulating factor 3 receptor (granulocyte) ELISA Kit;
Catalog
E010310
species
Human
GeneCSF3R
Standard Curve
Other Species Mouse Csf3r ELISA Kit
SpecificityNatural and recombinant human CSF3R
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human CSF3R in cell culture supernates, serum and plas
Intended UseHuman CSF3R ELISA Kit allows for the in vitro quantitative determination of CSF3R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human CSF3R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF3R has been precoated onto 96-well plates. Standards(NSO, E25-P621) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF3R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF3R amount of sample captured in plate. Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.
Human Granulocyte colony-stimulating factor receptor Protein information
Uniprot ID CSF3R_HUMAN
Uniprot AC Q99062;
UniGene Hs.524517;
GeneID 1441
KEGG hsa:1441;
Human Granulocyte colony-stimulating factor receptor Protein SEQUENCE
SEQUENCE 836 AA; 92156 MW; 3531ADDC979D4BC3 CRC64;
MARLGNCSLT WAALIILLLP GSLEECGHIS VSAPIVHLGD PITASCIIKQ
NCSHLDPEPQ ILWRLGAELQ PGGRQQRLSD GTQESIITLP HLNHTQAFLS
CCLNWGNSLQ ILDQVELRAG YPPAIPHNLS CLMNLTTSSL ICQWEPGPET
HLPTSFTLKS FKSRGNCQTQ GDSILDCVPK DGQSHCCIPR KHLLLYQNMG
IWVQAENALG TSMSPQLCLD PMDVVKLEPP MLRTMDPSPE AAPPQAGCLQ
LCWEPWQPGL HINQKCELRH KPQRGEASWA LVGPLPLEAL QYELCGLLPA
TAYTLQIRCI RWPLPGHWSD WSPSLELRTT ERAPTVRLDT WWRQRQLDPR
TVQLFWKPVP LEEDSGRIQG YVVSWRPSGQ AGAILPLCNT TELSCTFHLP
SEAQEVALVA YNSAGTSRPT PVVFSESRGP ALTRLHAMAR DPHSLWVGWE
PPNPWPQGYV IEWGLGPPSA SNSNKTWRME QNGRATGFLL KENIRPFQLY
EIIVTPLYQD TMGPSQHVYA YSQEMAPSHA PELHLKHIGK TWAQLEWVPE
PPELGKSPLT HYTIFWTNAQ NQSFSAILNA SSRGFVLHGL EPASLYHIHL
MAASQAGATN STVLTLMTLT PEGSELHIIL GLFGLLLLLT CLCGTAWLCC
SPNRKNPLWP SVPDPAHSSL GSWVPTIMEE DAFQLPGLGT PPITKLTVLE
EDEKKPVPWE SHNSSETCGL PTLVQTYVLQ GDPRAVSTQP QSQSGTSDQV
LYGQLLGSPT SPGPGHYLRC DSTQPLLAGL TPSPKSYENL WFQASPLGTL
VTPAPSQEDD CVFGPLLNFP LLQGIRVHGM EALGSF
UCSC uc001cav.3; human. [Q99062-1];

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Human CSF3R ELISA Kit