Human CRKL ELISA Kit

Alternative name
Human CRK like proto-oncogene, adaptor protein ELISA Kit;Human crk-like protein ELISA Kit;Human v-crk avian sarcoma virus CT10 oncogene homolog-like ELISA Kit;
Catalog
E010228
species
Human
GeneCRKL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Crk Like Protein (CRKL). No significant cross-reactivity or interference between Crk Like Protein (CRKL) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman CRKL ELISA Kit allows for the in vitro quantitative determination of CRKL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Crk-like protein Protein information
Uniprot ID CRKL_HUMAN
Uniprot AC P46109; A8KA44; D3DX35;
UniGene Hs.5613;
GeneID 1399
KEGG hsa:1399;
Human Crk-like protein Protein SEQUENCE
SEQUENCE 303 AA; 33777 MW; 294CF1EE2CD44B81 CRC64;
MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL
SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE
PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL
VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI
PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA
YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD
ENE
UCSC uc002ztf.2; human.;

Category

Human CRKL ELISA Kit

Human CRK like proto-oncogene, adaptor protein ELISA Kit;Human crk-like protein ELISA Kit;Human v-crk avian sarcoma virus CT10 oncogene homolog-like ELISA Kit;