Human CRHBP ELISA Kit

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  • Alternative name

    Human CRF-BP ELISA Kit;Human CRF-binding protein ELISA Kit;Human Corticotropin-releasing hormone-binding protein ELISA Kit;Human CRH-BP ELISA Kit;Human CRFBP ELISA Kit;Human corticotropin releasing hormone binding protein ELISA Kit;Human corticotropin-releasing factor-binding protein ELISA Kit;

  • Catalog
    E010224
  • species
    Human
  • GeneCRHBP
  • Other Species Mouse Crhbp ELISA KitRat Crhbp ELISA KitSheep CRHBP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CRHBP. No significant cross-reactivity or interference between Human CRHBP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CRHBP and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseHuman CRHBP ELISA Kit allows for the in vitro quantitative determination of CRHBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRHBP in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRHBP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CRHBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRHBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CRHBP. You can calculate the concentration of Human CRHBP in the samples by comparing the OD of the samples to the standard curve.
  • Human Corticotropin-releasing factor-binding protein Protein information
  • Uniprot ID CRHBP_HUMAN
  • Uniprot AC P24387; Q53F32; Q6FHT5;
  • UniGene Hs.115617;
  • GeneID 1393
  • KEGG hsa:1393;
  • Human Corticotropin-releasing factor-binding protein Protein SEQUENCE
  • SEQUENCE 322 AA; 36144 MW; 771A894BE06039BD CRC64;

    MSPNFKLQCH FILIFLTALR GESRYLELRE AADYDPFLLF SANLKRELAG

    EQPYRRALRC LDMLSLQGQF TFTADRPQLH CAAFFISEPE EFITIHYDQV

    SIDCQGGDFL KVFDGWILKG EKFPSSQDHP LPSAERYIDF CESGLSRRSI

    RSSQNVAMIF FRVHEPGNGF TLTIKTDPNL FPCNVISQTP NGKFTLVVPH

    QHRNCSFSII YPVVIKISDL TLGHVNGLQL KKSSAGCEGI GDFVELLGGT

    GLDPSKMTPL ADLCYPFHGP AQMKVGCDNT VVRMVSSGKH VNRVTFEYRQ

    LEPYELENPN GNSIGEFCLS GL

  • UCSC uc003ker.4; human.;


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