Human CRH ELISA Kit

Alternative name
Human corticotropin-releasing factor ELISA Kit;Human CRF ELISA Kit;Human Corticotropin-releasing hormone ELISA Kit;Human CRH1 ELISA Kit;Human corticotropin releasing hormone ELISA Kit;Human corticoliberin ELISA Kit;
Catalog
E010223
species
Human
GeneCRH
Other Species Mouse Crh ELISA Kit Mouse CRH-R ELISA Kit Bovine CRH ELISA Kit Canine CRH ELISA Kit Porcine CRH ELISA Kit Rat Crh ELISA Kit Sheep CRH ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CRH. No significant cross-reactivity or interference between Human CRH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CRH and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.375ng/mL
Intended UseHuman CRH ELISA Kit allows for the in vitro quantitative determination of CRH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a competitive enzyme immunoassay for in vitro quantitative measurement of CRH in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human CRH. During the reaction, Human CRH in the sample or standard competes with a fixed amount of Human CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human CRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Corticoliberin Protein information
Uniprot ID CRF_HUMAN
Uniprot AC P06850; B3KQS4;
UniGene Hs.75294;
GeneID 1392
KEGG hsa:1392;
Human Corticoliberin Protein SEQUENCE
SEQUENCE 196 AA; 21422 MW; 0CCDFF05BE364E92 CRC64;
MRLPLLVSAG VLLVALLPCP PCRALLSRGP VPGARQAPQH PQPLDFFQPP
PQSEQPQQPQ ARPVLLRMGE EYFLRLGNLN KSPAAPLSPA SSLLAGGSGS
RPSPEQATAN FFRVLLQQLL LPRRSLDSPA ALAERGARNA LGGHQEAPER
ERRSEEPPIS LDLTFHLLRE VLEMARAEQL AQQAHSNRKL MEIIGK
UCSC uc003xvy.3; human.;

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Category

Human CRH ELISA Kit

Human corticotropin-releasing factor ELISA Kit;Human CRF ELISA Kit;Human Corticotropin-releasing hormone ELISA Kit;Human CRH1 ELISA Kit;Human corticotropin releasing hormone ELISA Kit;Human corticoliberin ELISA Kit;