Human CKB ELISA Kit

Alternative name
Human B-CK ELISA Kit;Human creatine kinase B chain ELISA Kit;Human CKBB ELISA Kit;Human BCK ELISA Kit;Human HEL-211 ELISA Kit;Human HEL-S-29 ELISA Kit;Human creatine kinase B ELISA Kit;Human creatine kinase B-type ELISA Kit;Human creatine kinase brain ELISA Kit;Human creatine kinase brain-type ELISA Kit;Human epididymis luminal protein 211 ELISA Kit;Human epididymis secretory protein Li 29 ELISA Kit;
Catalog
E010188
species
Human
GeneCKB
Standard Curve
Other Species Mouse Ckb ELISA Kit Bovine CKB ELISA Kit Canine CKB ELISA Kit Chicken CKB ELISA Kit Porcine CKB ELISA Kit Rabbit CKB ELISA Kit Rat Ckb ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Brain (CKB). No significant cross-reactivity or interference between Creatine Kinase, Brain (CKB) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.31ng/mL.
Intended UseHuman CKB ELISA Kit allows for the in vitro quantitative determination of CKB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Brain (CKB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Brain (CKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Brain (CKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatine Kinase, Brain (CKB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Creatine kinase B-type Protein information
Uniprot ID KCRB_HUMAN
Uniprot AC P12277; A8K236; B2R5R4; Q2LE07; Q6FG40; Q9UC66;
UniGene Hs.173724;
GeneID 1152
KEGG hsa:1152;
Human Creatine kinase B-type Protein SEQUENCE
SEQUENCE 381 AA; 42644 MW; 637AA67A86AE3059 CRC64;
MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG
FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY
KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF
TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K

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Human CKB ELISA Kit